Effect of conjugate concentration on reactivity in the ES33-MICT. N, negative; P, positive; MAR value, the ratio of the RMU of test line divided by the RMU of control line; S/N, signal-to-noise ratio (the MAR value of the positive serum divided by the MAR value of the negative serum); 0.65, 0.65 μl of rES33-magnetic particle conjugate at a concentration of 10 mg/ml; 1, 1 μl of rES33-magnetic particle conjugate at a concentration of 10 mg/ml.