FIG. 1.

Cloning of viral genes into protein expression vectors. (A) The recombinant TAT-PRRSV-M and TAT-PRRSV-N genes were cloned into the pDrive and pCR2.1 cloning vectors, respectively. Once they were cloned into the cloning vectors, the insert DNAs were subcloned into the pQE30 and pQE30-UA E. coli expression vectors. (B) The porcine GM-CSF gene was cloned into the pcDNA3.1 mammalian protein expression vector. orf6 and orf7 of PRRSV cloned in the pCR2.1 vector were subcloned downstream of the GM-CSF gene.