FIG. 3.

Generation of monocyte-derived DCs. The levels of expression of DC markers were identified in the immature DCs (A) and mature DCs (B). The immature DCs were generated from monocytes by culturing PBMCs with GM-CSF and IL-4 for 6 days. The immature DCs became mature DCs as the result of 1 day of treatment with TNF-α. The levels of expression of MHC class I, MHC class II, CD172a, and CD80/CD86 molecules were determined by FACS analysis. The expression of MHC class I, MHC class II, and CD172a molecules was determined by the use of an FITC-conjugated monoclonal antibody specific for each corresponding molecule. The expression of CD80/CD86 was determined with an FITC-conjugated human CD152Ig protein. The x and y axes in each panel indicate the cell count and fluorescent intensity, respectively.