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. 2010 Feb 3;17(4):503–512. doi: 10.1128/CVI.00365-09

FIG. 5.

FIG. 5.

Identification of the recombinant genes and proteins expressed in mammalian cells. The mammalian expression vectors encoding the GM-CSF-PRRSV-M and GM-CSF-PRRSV-N proteins were transfected into 3T3 cells. After 2 days, RT-PCR and Western blotting were conducted to identify the expression of the recombinant genes and proteins in the cells. (A) RT-PCR for the detection of the recombinant GM-CSF-PRRSV-M gene. Lane 1, standard DNA marker; lane 2, cells transfected with the empty vector; lane 3, cells transfected with the pcDNA3.1-GM-CSF-PRRSV-M vector. (B) RT-PCR for the detection of the recombinant GM-CSF-PRRSV-N gene. Lane 1, standard DNA marker; lane 2, cells transfected with empty vector; lane 3, cells transfected with the pcDNA3.1-GM-CSF-PRRSV-N vector. (C) Western blotting for the detection of the recombinant GM-CSF-PRRSV-M protein. Lane 1, standard protein marker; lane 2, cells transfected with the empty vector; lane 3, cells transfected with the pcDNA3.1-GM-CSF-PRRSV-M vector. (D) Western blotting for the detection of the recombinant GM-CSF-PRRSV-N protein. Lane 1, standard protein marker; lane 2, cells transfected with the empty vector; lane 3, cells transfected with the pcDNA3.1-GM-CSF-PRRSV-N vector.