Antibody levels in the immunized pigs. Pigs were immunized with plasmid DNAs, and the levels antibodies to the PRRSV-M and PRRSV-N proteins were determined by ELISA. (A) Levels of antibody to the PRRSV-M protein in pigs immunized with pcDNA3.1-GM-CSF-PRRSV-M. Three pigs immunized with M protein (pigs M#1 to M#3, respectively) and two nonimmunized pigs (control pigs 1 and 2 [Cont#1 and Cont #2, respectively]) were examined. (B) Levels of antibody to PRRSV-N protein in pigs immunized with pcDNA3.1-GM-CSF-PRRSV-N. Three immunized pigs (N#1 to N#3, respectively) and two nonimmunized pigs (controls 1 and 2 [Cont#1 and Cont #2, respectively]) were assessed.