FIG. 8.

IFN-γ production from T cells. T cell-containing PBMCs were obtained from immunized pigs and nonimmunized pigs (control pigs C1 and C2). They were stimulated for 3 days by APCs at an effector cell-to-APC ratio of 5:1. PBMCs and DCs transduced with TAT-conjugated PRRSV-M or PRRSV-N proteins were used as APCs. Effector T cells from pigs immunized with the pcDNA3.1-GM-CSF-PRRSV-M (pigs M1 to M3) were stimulated by PBMCs (A) and DCs (C). Effector T cells from pigs immunized with pcDNA3.1-GM-CSF-PRRSV-N (pigs N1 to N3) were stimulated by PBMCs (B) and DCs (D). The amounts of IFN-γ in the cell culture supernatants were determined by ELISA. Statistical significance was determined by Student t test: **, P < 0.01; ***, P < 0.001.