TABLE 1.

Vectors and primers used to clone viral and cellular genes

Vector	Primer name	DNA sequence (5′ → 3′)	Product size (bp)
pCR2.1-TOPO	PRRSV-M FP1	GAA TTC ATG GGG TCG TCC TTA GAT GACa	537
	PRRSV-M RP1	GAT ATC TTA TTT GGC ATA TTT AAC AAGb
pCR2.1-TOPO	PRRSV-N FP1	GAA TTC ATG CCA AAT AAC AAC GGC AAG CAa	384
	PRRSV-N RP1	GAT ATC TCA GGC TGA GGG TGA TGC TGT Gb
pQE30-UA	PRRSV-M FP2	TAC TCA GCC ATA GAA ACC	270
	PRRSV-M RP2	TTA TTT GGC ATA TTT AAC AAG
pQE30-UA	PRRSV-N FP2	ATG CCA AAT AAC AAC GGC AAG CA	372
	PRRSV-N RP2	TCA GGC TGA GGG TGA TGC TGT G
pCR2.1-TOPO	PRRSV-N FP3	CTC GAG ATG CCA AAT AAC AAC GGC AAG CAc	384
	PRRSV-N RP3	TCT AGA TCA GGC TGA GGG TGA TGC TGT Gd
pCR2.1-TOPO	TAT FP	GGC AGG AAG AAG CGG AGA CAG CGA CGA AGA CCT CCT CAA TGC	42
	TAT RP	GCA TTG AGG AGG TCT TCG TCG CTG TCT CCG CTT CTT CCT GCC
pQE30	TAT-PRRSV-M FP	GGA TCC GGC AGG AAG AAG CGG AGA CAG CGA CGA AGA CCTe	324
		CCT CAA TGC TAC TCA GCC ATA GAA ACC
	TAT-PRRSV-M RP	GTC GAC TTA TTT GGC ATA TTT AACf
pQE30-UA	TAT-PRRSV-N FP	GGC AGG AAG AAG CGG AGA CAG CGA	414
	TAT-PRRSV-N RP	TCA GGC TGA GGG TGA TGC TGT G
pcDNA3.1	GM-CSF FP	GCC GCC GCC ATG GGG CTG CAG AAC CTG CTT Cg	447
	GM-CSF RP	GAA TTC CTT TTT GAC TGG CCC CCA GCA Aa

^aItalics indicate the EcoRI restriction enzyme site.

^bItalics indicate the EcoRV restriction enzyme site.

^cItalics indicate the XhoI restriction enzyme site.

^dItalics indicate the XbaI restriction enzyme site.

^eItalics indicate the BamHI restriction enzyme site.

^fItalics indicate the SalI restriction enzyme site.

^gItalics indicate the Kozak sequence.