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. 2010 Jan 27;17(4):660–667. doi: 10.1128/CVI.00358-09

FIG. 1.

FIG. 1.

Western blot analysis of the 115-kDa protease of L. donovani. Cell-free cultured supernatants from early-passage promastigotes and whole-cell extracts from virulent, avirulent, and attenuated strains of L. donovani were loaded directly onto an SDS gel and used for immunoblot detection with the anti-115-kDa serine protease antibody. Lane a, purified protease (2 μg protein) from the culture supernatant of L. donovani; lane b, whole-cell extract of early-passage (4th-P) L. donovani promastigotes (100 μg protein); lane c, whole-cell extract of late-passage (34th-P) L. donovani promastigotes (100 μg protein); lane d, whole-cell extract of UR6 promastigotes (100 μg protein). Actin was used as a loading control to normalize the data.