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Intracellular localization of the 115-kDa serine protease of L. donovani by immunogold electron microscopy. Promastigotes (A) and amastigotes (B) of L. donovani were labeled with an anti-115-kDa serine protease antibody prior to detection with a gold-conjugated secondary antibody. Electron microscopy images show the presence of gold particles in the flagellar pocket regions of the parasites (arrows).
