TABLE 1.
Characterization of the immune response elicited by OpaJ-containing liposomes adjuvanted with LpxL1 or PagL LPS as measured in ELISAs
| Group | LPS dose (μg) | Anti-OpaJ titera |
Nonspecific IgG titerb | Nonspecific IgM titerb | |||||
|---|---|---|---|---|---|---|---|---|---|
| IgG | IgG1 | IgG2a | IgG2b | IgG3 | IgM | ||||
| Not adjuvanted | 3.5 | 3.4 | 0.4 | 0.8 | 0.3 | 2.1 | 2.2 | 1.7 | |
| AlPO4 | 3.6 | 3.6 | 0.8 | 1.9 | 1.0 | 2.5 | 0.6 | 1.5 | |
| LpxL1 coincorporated | 1 | 4.0* | 4.3* | 2.0*§ | 2.7* | 1.8 | 0.6 | 0.6 | 0.6 |
| Free LpxL1 | 1 | 4.1* | 4.2 | 3.0*§ | 3.2* | 1.0 | 1.8 | 0.6 | 1.6 |
| PagL coincorporated | 1 | 3.8 | 3.4 | 2.7* | 2.9* | 1.3 | 0.6 | 0.3 | 0.6 |
| Free PagL | 1 | 3.8 | 4.1 | 3.2* | 3.1* | 1.3 | 2.3 | 0.6 | 1.7 |
| PagL coincorporated | 0.1 | 3.9 | 3.9 | 3.0* | 3.0*§ | 1.3 | 1.0 | 0.6 | 0.3 |
| Free PagL | 0.1 | 4.2* | 4.1 | 3.3* | 3.7*§ | 1.0 | 1.6 | 0.6 | 0.8 |
The titer of each anti-OpaJ Ig was determined by whole-cell ELISA in plates coated with OpaJ+ cells of N. meningitidis strain H44/76 of immunotype L3. Data are expressed as the mean log10 titer. The results for the different groups were compared by ANOVA with a confidence level of 95% when appropriate. *, statistically significant differences compared with the nonadjuvanted group (P < 0.05). §, statistically significant differences between groups adjuvanted with free LPS or LPS coincorporated with OpaJ into the liposomes (P < 0.05).
The titer of each antiserum was determined by whole-cell ELISA in plates coated with OpaJ− cells of N. meningitidis strain H44/76 of immunotype L3. Data are expressed as the mean log10 titer.