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Construction of hybrid NRPS using λ Red-mediated recombination. In this example, domains of the d-Lys8 module were deleted and replaced by a selectable marker, which was in turn replaced by the corresponding domains of the d-Asn11 module cloned by the gap repair technique (36). The process results in the fusion of LptB and LptC and the elimination of the LptB stop codon.
