TABLE 2.
Strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BAS849 | ΔlamB106 imp-4213 | 39 |
| BW25113 | laclqrrnBT14 ΔlacZWJ16hsdR514 | 13 |
| ML22 | DH10B::pUZ8002 | 11 |
| E. faecalis | ||
| 201 | CLSI test strain | ATCC 49452 |
| 312 | E. faecalis 201 mutant with reduced susceptibility to Dap | Cubist strain collection |
| E. faecium | ||
| 14 | CLSI test strain | ATCC 6569 |
| 384 | E. faecium 14 mutant with reduced susceptibility to Dap | Cubist strain collection |
| S. aureus | ||
| 42 | CLSI test strain | ATCC 29213 |
| 339 | CLSI clinical MRSA isolate | ATCC 43300 |
| MW2 | MRSA; genomic sequence available | 2 |
| 1616 | MW2 with reduced susceptibility to Dap | 17 |
| 1695 | MW2 with reduced susceptibility to Dap | 17 |
| S. pneumoniae 402 | CLSI test strain | ATCC 6303 |
| S. fradiae | ||
| XH25 | rpsL; producer of A54145 | Alexander et al., submitted |
| DA613 | XH25 ΔlptI::tsr | Alexander et al., submitted |
| DA740 | XH25 ΔlptBCD | Alexander et al., submitted |
| DA901 | XH25 ΔlptBCD ΔlptI::tsr | Alexander et al., submitted |
| DA1187 | XH25 ΔlptEFABCDGHJKLMNPI | Alexander et al., submitted |
| XH1000 | DA1187::pKN55 | This study |
| KN707 | DA901::pKN56 | This study |
| KN681 | DA740::pKN56 | This study |
| KN715 | DA901::pKN57 | This study |
| KN689 | DA740::pKN57 | This study |
| KN723 | DA901::pKN58 | This study |
| KN697 | DA740::pKN58 | This study |
| KN728 | DA901::pKN59 | This study |
| KN701 | DA740::pKN59 | This study |
| KN730 | DA901::pKN60 | This study |
| KN705 | DA740::pKN60 | This study |
| KN649 | DA1187::pKN61 | This study |
| KN665 | XH1000::pKN61 | This study |
| KN661 | DA1187::pKN63 | This study |
| KN677 | XH1000::pKN63 | This study |
| XH1003 | DA1187::pXH11 | This study |
| XH1035 | XH1000::pXH11 | This study |
| Plasmids | ||
| pDA2048 | pECBAC1-derived; oriT att/intφC31Amr::ermEp*lptBCDGH | Alexander et al., submitted |
| pDA2054 | pECBAC1-derived; oriT att/intφC31Amr::lptEFABCDGHJKLMNP | Alexander et al., submitted |
| pRT802 | Kanratt/intφBT1 | 19 |
| pKN54 | pRT802::ermEp* | This study |
| pKN55 | pKN54::dptIJ | This study |
| pKN56 | pDA2048 ΔD-Lys8-CAT::D-Ala-CAT | This study |
| pKN57 | pDA2048 ΔD-Lys8-CAT::D-Ser-CAT | This study |
| pKN58 | pDA2048 ΔD-Lys8-CAT::D-Asn-CAT | This study |
| pKN59 | pDA2048 ΔD-Asn11-CAT::D-Ala-CAT | This study |
| pKN60 | pDA2048 ΔD-Asn11-CAT::D-Ser-CAT | This study |
| pKN61 | pDA2054 ΔD-Glu2-D-Lys8::D-Asn2-D-Ala8 | This study |
| pKN63 | pDA2054 ΔD-Glu2-Thr4::D-Asn2-D-Thr4 | This study |
| pXH11 | pDA2054 ΔD-Glu2-CAT::D-Asn2-CAT | This study |