FIG. 1.
Time-kill curves of C. glabrata wild-type and mutant strains exposed to fluconazole. Logarithmic-phase cells (5 × 105 CFU/ml) were incubated in minimal medium with agitation in the presence or absence of fluconazole at the indicated concentrations. The total number of cells was counted using a hemocytometer, and the number of viable cells was determined by plating the appropriate dilutions on yeast extract-peptone-dextrose (YPD) plates. The data are expressed as the percentages of viability and represent the means and standard deviations for three independent experiments.