FIG. 3.
Virulence assay using a mouse model of disseminated candidiasis. Groups of 10 mice were intravenously inoculated with 8 × 107 cells for each C. glabrata strain. Three target organs (liver, spleen, and bilateral kidneys) were excised 7 days after injection. Appropriate dilutions of organ homogenates were plated, and the numbers of CFU were counted after 3 days of incubation at 30°C. Numbers of recovered CFU from each organ are indicated for individual mice in the scatter plots. The geometric mean is shown as a bar. Representative data of two independent experiments are shown. C. glabrata strains are as follows: wild type, strain TG11 (wild-type control) (filled circles); Δcnb1, TG162 (Δcnb1 strain containing an empty vector) (squares); Δcnb1 + CNB1, TG163 (CNB1-complemented strain made with pCgACT-PNB) (triangles); Δcrz1, TG172 (Δcrz1 strain containing an empty vector) (inverted triangles); Δcrz1 + CRZ1, TG173 (CRZ1-complemented strain made with pCgACT-PRZ) (diamonds). ¶, P < 0.05 (Kruskal-Wallis test with Dunn's posttest).