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. 2010 Jan 25;54(4):1492–1497. doi: 10.1128/AAC.01495-09

FIG. 1.

FIG. 1.

Western blotting of periplasmic materials extracted from cells expressing hybrid MexA proteins. Whole-cell lysate (4 μg protein) (lanes 1 to 5) and periplasmic materials (1 μg protein) (lanes 6 to 10) were subjected to SDS-PAGE (12% gel), blotted onto a polyvinylidene fluoride membrane, and visualized with anti-MexA antibody. Lanes 1 and 6, PAO4290; lanes 2 and 7, TNP070; lanes 3 and 8, TNP070(pPlcMexA); lanes 4 and 9, TNP080(pPlcMexA); lanes 5 and 10, TNP080(pTatPlcMexA). The white and black arrowheads indicate mature and unprocessed MexA.