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Absorption spectra of culture supernatants. Cells were grown in Casamino Acid medium overnight at 37°C under aeration by reciprocal shaking, and the culture was centrifuged at 13,700 × g for 10 min. The clear supernatant was scanned with a spectrophotometer (V-630 Bio; Jasco Co., Japan). (a) PAO4290. (b) TNP070. (c) TNP080. (d) TNP080(pTatPlcMexA).
