TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant propertiesa | Reference or source |
|---|---|---|
| Strains | ||
| E. coli JM109 | recA1 endA1 gyrA96 thi hsdR17(rK−rK+) e14− (mcrA−) supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+lacIqlacZΔM15] | Laboratory strain |
| E. coli S17-1 | RP4-2-Tc::Mu-Km::Tn7 pro res−mod+ | 26 |
| P. aeruginosa PA04290 | leu-10 argF10 aph-9004 FP− | Matsumoto collection |
| P. aeruginosa TNP070 | ΔmexA derivative of PAO4290 | 35 |
| P. aeruginosa TNP080 | ΔtatC derivative of TNP070 | This study |
| Plasmids | ||
| pBluescript II SK(+) | Cloning vector; Apr | Stratagene |
| pUC18 | Cloning vector; Apr | Takara |
| pTH18ks1 | pSC101 derivative; repAts1 Kmr | 9 |
| pK18mob | pK18 derivative carrying RP4 mob region; Kmr | 25 |
| pMMB67HE | Broad-host-range vector; Apr IncQ | 8 |
| pMEXA1 | pMMB67HE derivative carrying wild-type mexA gene; Apr | 34 |
| pBlueMexA | pBluescript II SK(+) derivative carrying mexA gene; Apr | This study |
| pTH18-PlcMexA | pTH18ks1 derivative carrying chimeric mexA gene with plcH signal sequence; Kmr | This study |
| pPlcMexA | pMMB67HE derivative carrying chimeric mexA gene with plcH signal sequence; Apr | This study |
| pK18ΔTat | pK18mob derivative carrying internal tatC gene and mob gene; Kmr | This study |
| pTH18-TatPlcMexA | pTH18ks1 derivative carrying entire tatC gene and the chimeric mexA gene; Kmr | This study |
| pTatPlcMexA | pMMB67HE derivative carrying entire tatC gene and the chimeric mexA gene; Apr | This study |
a
Ap, ampicillin; Km, kanamycin.