TABLE 2.
Primer | Sequencea | Tag |
---|---|---|
PlcH-F | 5′-GAGGATCCTCGCTGTATTCCCTGCC-3′ | BamHI |
PlcH-R | 5′-CGGCGCCTCGCTTTTTCCCTCGACGGCCAGGGCGCG-3′ | |
MexA-F | 5′-CGCGCCCTGGCCGTCGAGGGAAAAAGCGAGGCGCCG-3′ | |
MexA-R | 5′-AGGGTACCCTTGATCAGCCCTTGC-3′ | KpnI |
TatC-F1 | 5′-GTGGATCCACCTGACCGAACTGCGTACG-3′ | BamHI |
TatC-R1 | 5′-ACGGATCCGTCAGGACGAAGTCCTAGGTAG-3′ | BamHI |
TatC-F2 | 5′-GTGGATCCACCTGACCGAACTGCGTACG-3′ | BamHI |
TatC-R2 | 5′-ACGGATCCGTCAGGACGAAGTCCAGGTAG-3′ | BamHI |
Restriction site tags are underlined.