FIG. 1.
LPS from F. tularensis LVS or Schu S4 does not induce expression of E-selectin by HUVEC. HUVEC were incubated for 4 h at 37°C with medium alone (Unstim), 1 or 10 μg of F. tularensis LVS LPS/ml, 1 or 10 μg of F. tularensis Schu S4 LPS/ml, or 0.0002 μg of E. coli (Ec) LPS/ml. The levels of E-selectin were determined by whole-cell ELISA. Bars represent the means ± the standard deviations (SD) of four replicate samples. *, Significantly greater than the unstimulated samples (P < 0.001). This experiment was repeated two more times, yielding similar results.