TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| V. parahaemolyticus strains | ||
| RIMD2210633 (WT) | Clinical isolate; KP positive; serotype O3:K6 | 27 |
| ΔvscN1 | T3SS1-deficient strain; vscN1 deletion mutant (deletion from nt −225 to 1068 of the gene) derived from WT | This study |
| ΔvscN2 | T3SS2-deficient strain; in-frame vscN2 deletion mutant (deletion from nt 160 to 978 of the gene) derived from WT | This study |
| ΔvscN1 ΔvscN2 | T3SS1- and T3SS2-deficient strain; vscN1 (deletion from nt −225 to 1068 of the gene) and vscN2 (deletion from nt 160 to 978 of the gene) double-deletion mutant derived from WT | This study |
| POR-1 | tdhAS null mutant strain; tdhAS deletion mutant derived from WT | 32 |
| POR-2 | TDH- and T3SS1-deficient strain; in-frame vcrD1 deletion mutant (deletion from nt 640 to 1731 of the gene) derived from POR-1 | 33 |
| POR-3 | TDH- and T3SS2-deficient strain; in-frame vcrD2 deletion mutant (deletion from nt 315 to 761 of the gene) derived from POR-1 | 33 |
| ΔvcrD1 ΔvcrD2 | TDH-, T3SS1-, and T3SS2-deficient strain; vcrD1 (deletion from nt 640 to 1731 of the gene) and vcrD2 (deletion from nt 315 to 761 of the gene) double-deletion mutant derived from POR-1 | 19 |
| E. coli strains | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 phoA supE44 thi-1 gyrA96 relA1 λ− | Novagen |
| SM10 λpir | thi thr leu tonA lacY supE recA::RP4-2Tc::Mu λpir R6K | 24 |
| Plasmids | ||
| pT7bule | Multicopy (ColE1 ori) TA cloning vector, Ampr | Novagen |
| pYAK1 | R6K-ori suicide vector for gene replacement, Cmr | 17 |
| pYAK1-ΔvscN1 | Derivative of suicide vector pYAK1 for generating the vscN1 deletion mutant | 33 |
| pYAK-ΔvscN2 | Derivative of suicide vector pYAK1 for generating the vscN2 deletion mutant | 33 |
| pSA19Cm-MCS | Complement vector for V. parahaemolyticus, Cmr | 18 |
| pSACm-1 | pSA19Cm-MCS containing promoter of vopN1-vopD1 operon in EcoRI-BamHI site | 31 |
| pSA-tdhP | pSA19Cm-MCS containing promoter of tdhA in EcoRI-SmaI site | 18 |
| ptdhA | Derivative of pSACm-MCS containing tdhA loci | This study |
| pvcrD1 | Derivative of pSACm-1 containing vcrD1 loci | This study |
| pvscN2 | Derivative of pSA-tdhP containing vscN2 loci | This study |