FIG. 4.
Time course of the intoxication of HeLa cells with C2 toxin possessing the long (C2IIa) or short (ΔC2IIa) version of the binding component. HeLa cells were treated with 430 pM C2I and 1 nM C2IIa or ΔC2IIa. As a control, HeLa cells were treated with 430 pM C2I alone. Cells were fixed after the indicated times, and the actin cytoskeleton was stained by TRITC-phalloidin (red). The nuclei were stained by DAPI (blue). Bars, 30 μm.