FIG. 3.

Analysis of injected and total cells recovered from the lungs of mice infected with P. aeruginosa. FACS analysis was performed with cells recovered from the whole lungs of mice infected with PA99null+ExoU(S142A)-Bla at 18 h postinfection. (A and B) Numbers of injected cells (A) and total cells (B) for each immune cell type recovered from whole lungs per mouse. The numbers of cells per mouse were determined by equating the number of total cells with normal scatter characteristics measured by flow cytometry to the number of trypan blue-negative cells counted with the hemacytometer. (C) Proportion of each of the cell subpopulations purified from the lungs that was injected with ExoU(S142A)-Bla. A significantly higher proportion of neutrophils than of monocytes, macrophages, dendritic cells, or lymphocytes was injected. The data are means ± standard errors of the means (≥3 mice per group); similar results were obtained in at least three independent experiments. The results varied from day to day, and the mean percentage of injected cells ranged from 7 to 15%. *, P < 0.01.