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. 2010 Jan 25;78(4):1447–1456. doi: 10.1128/IAI.01134-09

FIG. 6.

FIG. 6.

Few type II alveolar epithelial cells (pneumocytes) are injected with ExoU(S142A). FACS analysis was performed with type II pneumocytes isolated from lungs of mice infected with PA99null+ExoU(S142A)-Bla at 18 h postinfection. Cells recovered by BAL were analyzed in parallel. (A) Histogram showing blue fluorescence of cells from BAL fluid (top panel) or type II pneumocyte preparations (bottom panel). Blue cells indicate injection with ExoU(S142A)-Bla. Brackets indicate cells exhibiting blue fluorescence. Representative plots from a total of three experiments are shown. (B) Percentages of cells in BAL fluid or type II pneumocyte preparations that were injected with ExoU(S142A)-Bla. The data are means ± standard errors of the means for triplicate samples of cells pooled from three mice; similar results were obtained in three independent experiments. *, P < 0.00001.