Prolonged restraint had little effect on measures of mucosal immunity. (A) Colonic IgA levels were unaffected by restraint exposure prior to challenge. n = 3 per group prechallenge; n = 5 per group on days 1, 6, 12, and 24 postchallenge. (B) Gene expression for mouse β-defensin 1 was similar in RST mice and in HCCl mice, with FWD control mice having lower gene expression on day 6 postchallenge (*, P < 0.05). n = 5 per group prechallenge; n = 3 per group on days 1, 6, 12, and 24 postchallenge. (C) TNF-α levels were suppressed in RST mice compared to HCC mice on days 12 and 24 postchallenge (*, P < 0.05 versus HCC mice). n = 3 per group prechallenge; n = 5 per group on days 1, 6, 12, and 24 postchallenge. In all cases the data are the means ± standard errors.