TABLE 1.
Primer and probe 5′-3′ sequences
| Gene | Sequence |
||
|---|---|---|---|
| Forward | Reverse | Probe | |
| 18S | CGGCTACCACATCCAAGGAA | GCTGGAATTACCGCGGCT | TGCTGGCACCAGACTTGCCCTC |
| IL-1β | GGCCTCAAAGGAAAGAATCTATACC | GTATTGCTTGGGATCCACACTCT | ATGAAAGACGGCACACCCACCCTG |
| iNOS | CAGCTGGGCTGTACAAACCTT | TGAATGTGATGTTTGCTTCGG | CGGGCAGCCTGTGAGACCTTTGA |
| TNF-α | CTGTCTACTGAACTTCGGGGTGAT | GGTCTGGGCCATAGAACTGATG | TGAGAAGTTCCCAAATGGCCTCCCTC |
| IL-10 | TTTGAATTCCCTGGGTGAGAA | ACAGGGGAGAAATCGATGACA | NAa |
| Mouse β-defensin 1 | GGCTGCCACCACTATGAAAACTC | GAGACAGAATCCTCCATGTTGAA | NA |
| Mouse β-defensin 3 | GCATTTGAGGAAAGGAACTCCACAAC | GTCTCCACCTGCAGCTTTTAGCAA | NA |
a
NA, not applicable because SYBR green was used in place of a fluorescent probe.