TABLE 2.

Diversity analysis based upon rarefaction, OTU, ACE, Chao1, and modeling of rarefaction to predict maximum OTU^a

Group	Diversity								Maximum modeled OTU species
	Rarefaction		OTU		ACE		Chao1
	Species	Genus	Species	Genus	Species	Genus	Species	Genus
Baseline	588	387	601	393	1,102	572	1,011	545	621
FWD D1	451	295	456	297	741	376	738	373	478
FWD D3	529	345	531	346	843	435	836	438	571
FWD D5b	598	374	602	376	992	488	968	493	667
FWD D7b	534	343	537	344	874	435	854	419	577
RST D1	534	324	539	326	876	402	807	395	588
RST D3	458	304	461	305	756	402	746	413	485
RST D5b	468	298	470	299	776	369	763	368	501
RST D7b	413	267	415	268	633	320	646	312	438

^aSpecies designations are based upon 3% sequence divergence, and genus designations are based upon 5% sequence divergence. The data are the means from 3 animals per treatment, i.e., food and water deprivation control (FWD) and restraint stressor (RST), at each time point (i.e., after D1, D3, D5, and D7 under each condition).

^bBased upon Student's t test, there is a significant difference in the predictions between FWD D5 and D7 and RST D5 and D7 (P < 0.05) for all estimations.