TABLE 5.
Strain or solution | Plasmid(s) | Size of inoculum (log CFU) | No. of chickens colonized/total no. |
Lung |
Spleen |
Liver |
||||
---|---|---|---|---|---|---|---|---|---|---|
Air sacs | Pericardial fluid | No. of chickens colonized/total no. | Mean no. of bacteria (log10 CFU/g)a | No. of chickens colonized/total no. | Mean no. of bacteria (log10 CFU/g)a | No. of chickens colonized/total no. | Mean no. of bacteria (log10 CFU/g)a | |||
χ7122 | pAPEC-1, pAPEC-2, pAPEC-3 | 7.2 | 11/12 | 12/12 | 12/12 | 3.10 ± 0.94 | 8/8 | 4.01 ± 0.57 | 7/8 | 3.06 ± 1.57 |
χ7368 | None | 7.1 | 1/14 | 03/14 | 01/14 | 0.19 ± 0.72c | 2/10 | 0.74 ± 1.06c | 1/10 | 0.12 ± 0.45d |
χ7394b | pAPEC-1 | 7.1 | 11/12 | 11/12 | 10/12 | 3.28 ± 2.03 | 7/8 | 3.65 ± 1.71 | 6/8 | 2.41 ± 2.04 |
χ7392 | pAPEC-2 | 7.1 | 3/14 | 00/14 | 02/14 | 0.49 ± 1.25c | 2/10 | 0.62 ± 1.34c | 0/10 | 0.16 ± 0.59d |
χ7367 | pAPEC-3 | 7.0 | 0/14 | 00/14 | 03/14 | 0.51 ± 1.12d | 2/10 | 0.59 ± 1.31c | 0/10 | 0.12 ± 0.45d |
χ7561 | pAPEC-1, pAPEC-2 | 7.3 | 12/14 | 14/14 | 12/14 | 2.76 ± 1.84 | 9/10 | 2.98 ± 1.23 | 5/10 | 1.92 ± 1.59 |
χ7562b | pAPEC-1, PAPEC-3 | 7.2 | 12/13 | 12/13 | 12/13 | 4.29 ± 1.87e | 8/8 | 4.28 ± 0.80 | 7/8 | 3.45 ± 1.70 |
χ7274 | pAPEC-2, pAPEC-3 | 7.4 | 04/14 | 06/14 | 01/14 | 0.18 ± 0.68c | 2/10 | 0.80 ± 1.35c | 0/10 | 0.00 ± 0.00d |
PBS | 0 | 0/8 | 0/8 | 0/8 | 0.00 ± 0.00 | 0/8 | 0.00 ± 0.00 | 0/8 | 0.00 ± 0.00 |
The values are means ± standard deviations for 8 or 14 birds from each group. Counts were determined at 48 h postinoculation.
Two chickens died between 24 and 48 h after inoculation.
Significantly different from the wild type (P < 0.001).
Significantly different from the wild type (P < 0.0001).
Significantly different from the wild type (P = 0.004).