TABLE 1.
2D-DIGE data for pI 4 to 7
| Protein | Locusa | Mol wt (103)b | pIb | Combined MS and MS/MS search scoresc | No. of matched peptides (no. of unmatched peptides) | No. of peptides with MS/MS data | % of amino acids accounted for by matching peptides (coverage) | Avg wild-type/fur mutant volume ratio | Pd |
|---|---|---|---|---|---|---|---|---|---|
| Lipase (LipA) | NWMN_2569 | 76.7 | 77.0 | 7103 | 15 | 73 | 24 | −4.24 | 0.00038 |
| N-Acetylmuramoyl-l-alanine amidase | NWMN_2543 | 69 | 6.0 | 114 | 16 | 3 | 32 | −4.41 | 9.60E−04 |
| Coagulase (Coa) | NWMN_0166 | 71.6 | 8.4 | 131 | 13 | 5 | 34 | 1.88 | 3.10E−03 |
| Dihydrolipoamide dehydrogenase (PdhD) | NWMN_0962 | 49.5 | 4.95 | 68 | 10 | 1 | 22 | 2.47 | 0.0038 |
| 6-Phosphogluconate dehydrogenase | NWMN_1417 | 52 | 5.0 | 77 | 5 | 0 | 11 | 1.97 | 3.50E−03 |
| Phosphoglycerate kinase | NWMN_0742 | 42.6 | 5.2 | 79 | 7 | 3 | 23 | 3.54 | 2.10E−05 |
| Glyceraldehyde 3-phosphate dehydrogenase 1 (GapA) | NWMN_0741 | 36.4 | 4.9 | 245 | 14 | 8 | 39 | 4.08 | 1.30E−05 |
| l-Lactate dehydrogenase (Ldh) | NWMN_2499 | 34.5 | 4.8 | 62 | 4 | 3 | 13 | 5.60 | 1.90E−04 |
| Elongation factor TS | NWMN_1167 | 32.5 | 5.2 | 177 | 12 | 5 | 46 | 2.74 | 0.001 |
| Alcohol dehydrogenase I | NWMN_0577 | 36.4 | 5.5 | 52 | 5 | 3 | 18 | 4.53 | 7.30E−05 |
| Cysteine synthase | NWMN_0475 | 33 | 5.2 | 43 | 3 | 2 | 11 | 2.73 | 6.10E−04 |
| 1-Phosphatidylinositol phosphodiesterase (PI-PLCc) | NWMN_0041 | 35 | 6.5 | 78 | 10 (1) | 1 | 26 | −3.00 | 1.40E−03 |
| Triose phosphate isomerase | NWMN_0743 | 27.4 | 4.8 | 71 | 5 (1) | 3 | 23 | 2.25 | 9.70E−05 |
| Secretory antigen SsaA homolog | NWMN_0634 | 28.2 | 6.1 | 46 | 3 (2) | 1 | 14 | −1.71 | 0.00071 |
| Ssl 11 | NWMN_0400 | 25.3 | 8.5 | 135 | 13 | 6 | 66 | 4.45 | 2.20E−05 |
| Ssl 1e | NWMN_0388 | 25.6 | 8.5 | 146 | 15 (3) | 5 | 54 | 11.61 | 6.00E−05 |
| Ssl 2e | NWMN_0389 | 25 | 9.0 | 67 | 7 | 1 | 30 | 11.61 | 6.00E−05 |
| Alkyl hydroperoxide reductase subunit C (AphC) | NWMN_0372 | 21 | 4.9 | 90 | 5 | 3 | 40 | −3.06 | 4.40E−05 |
| Hypothetical protein | NWMN_0272 | 21.4 | 5.3 | 82 | 5 | 1 | 25 | 1.48 | 0.026 |
| FLIPr | NWMN_1067 | 15.2 | 9.1 | 256 | 10 (1) | 5 | 48 | 4.27 | 5.90E−06 |
a
Gene annotation in the NCBI database for S. aureus strain Newman.
b
The theoretical molecular weights and isoelectric points were calculated using the database entries, which often contain precursor sequences not present in the mature form migrating on the gel.
c
Combined MS and MS/MS search (MOWSE) scores greater than 79 are within the 95% confidence interval. Scores were calculated using the MASCOT v1.9 database search algorithms.
d
P values determined by Student's t test. Analysis of variance (ANOVA) P values were calculated using DeCyder software version 6.5 and the mixed-sample internal standard methodology.
e
The sample used was a mixed sample containing peptides for Ssl 1 and Ssl 2.