TABLE 2.
Kd and IC50s of GSTs for heme-related compoundsa
| Recombinant GST |
Kd value (μM) for: |
IC50 (μM) for: |
||
|---|---|---|---|---|
| Hematin | Protoporphyrin IX | Hematin | Protoporphyrin IX | |
| Na-GST-1 | 3.970 ± 0.3973 | 25.44 ± 1.415 | 0.23 ± 0.01 | 324 ± 15 |
| Na-GST-2 | 2.905 ± 0.1174 | 24.38 ± 1.322 | 0.07 ± 0.01 | 387 ± 18 |
| Na-GST-3 | 3.691 ± 0.2388 | 13.78 ± 0.5693 | 0.32 ± 0.04 | 14 ± 1 |
| Ac-GST-1 | 2.174 ± 0.1948 | 54.38 ± 1.596 | 0.38 ± 0.01 | |
a
Values are the averages ± standard deviations of 4 replicates. Dissociation constants (Kd) for heme-related compounds were determined following quenching of intrinsic fluorescence of recombinant hookworm GSTs, and IC50s of heme-related compounds were determined for hookworm GST-catalyzed GSH-CDNB conjugation.