TABLE 1.
Transposon mutagenesis in M. agalactiae
| Transformation no.a | No. of clones isolated | No. (%) of mutants in: |
|
|---|---|---|---|
| Group Ab | Group Bc | ||
| T01 | 52 | 0 (0.0) | 5 (9.6) |
| T02 | 65 | 0 (0.0) | 8 (12.3) |
| T05 | 149 | 8 (5.4) | 30 (20.1) |
| T06 | 169 | 3 (1.8) | 35 (20.7) |
| T07 | 175 | 2 (1.1) | 1 (0.6) |
| T08 | 270 | 2 (0.7) | 23 (8.5) |
| T09 | 247 | 1 (0.4) | 47 (19.0) |
| T10 | 686 | 7 (1.0) | 27 (3.9) |
| Total | 1,813 | 23 (1.3) | 176 (9.7) |
a
Independent transformation assays with plasmid pMT85.
b
Growth-deficient mutants in cell culture.
c
Mutants found to be moderately inhibited upon high-throughput screening of the mutant library in cell culture.