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Representative Western blot assay showing the range of signals detected when whole-cell lysates of piliated K. kingae clinical isolates were examined for PilA1 reactivity using antiserum GP65 raised against PilA1 from K. kingae strain 269-492. Strain 269-492 was used as a positive control, and strain 269-492 pilA1::aphA3 was used as a negative control.
