TABLE 1.
Analysis of the clinical isolates used in this study
| Straine | Site/yr of isolation | Disease state | Colony typea | Pilib | Adherence to Chang/Hig-82c | PilA1 expressiond |
|---|---|---|---|---|---|---|
| PYIDO | Blood/2007 | Tenosynovitis | SC | + | +/+ | + |
| PYKH-B | Blood/2007 | Septic arthritis | NS/NC | + | +/+ | ++++ |
| PYHER | Blood/2005 | Osteomyelitis | D | − | −/− | NAg |
| PYITK-B | Blood/2007 | Croup | SC | + | +/+ | ++ |
| PYKK096 | Blood/1992 | Croup | NS/NC | + | +/+ | − |
| PYKK060 | Blood/1994 | Endocarditis | NS/NC | + | +/+ | +++ |
| PYKK190 | Blood/2002 | Endocarditis | D | − | −/− | NA |
| PYKK197 | Blood/2003 | Endocarditis | D | − | −/− | NA |
| PYKK199 | Blood//2004 | Endocarditis | D | − | −/− | NA |
| PYKK061 | Blood/1995 | Bacteremia | SC | + | +/+ | − |
| PYKK092 | Blood/1996 | Bacteremia | D | − | −/− | NA |
| PYKK093 | Blood/1995 | Bacteremia | NS/NC | + | +/+ | − |
| PYKK097 | Blood/1992 | Bacteremia | NS/NC | + | +/+ | − |
| PYKK128 | Blood/1997 | Bacteremia | D | − | −/− | NA |
| PYKK129 | Blood/1997 | Bacteremia | NS/NC | + | +/+ | ++++ |
| PYKK181 | Blood/2002 | Bacteremia | NS/NC | + | +/+ | + |
| PYKK243 | Blood/2005 | Bacteremia | SC | + | +/+ | ++ |
| PYKK079 | Blood/1994 | Osteomyelitis | NS/NC | + | +/+ | − |
| PYIDO PH-1 | Respiratory/2007 | Tenosynovitis | SC | + | +/+ | + |
| PYKH PH-1 | Respiratory/2007 | Septic arthritis | NS/NC | + | +/+ | ++++ |
| PYITK PH-1 | Respiratory/2007 | Croup | SC | + | +/+ | ++ |
| PYITK PH-2 | Respiratory/2007 | Croup | SC | + | +/+ | ++ |
| PYAA026 | Respiratory/2006 | Carrier | SC | + | +/+ | ++++ |
| PYAA417 | Respiratory/2006 | Carrier | NS/NC | + | +/+ | ++ |
| PYBB285 | Respiratory/2006 | Carrier | SC | + | +/+ | ++ |
| PYC1639 | Respiratory/2006 | Carrier | SC | + | +/+ | + |
| PYKK002 | Respiratory/1994 | Carrier | NS/NC | + | +/+ | ++ |
| PYKK003 | Respiratory/1994 | Carrier | D | −f | −/− | − |
| PYKK012 | Respiratory/1994 | Carrier | SC | + | +/+ | ++++ |
| PYKK019 | Respiratory/1993 | Carrier | D | − | −/− | NA |
| PYKK021 | Respiratory/1993 | Carrier | D | − | −/− | NA |
| PYKK029 | Respiratory/1994 | Carrier | NS/NC | + | +/+ | − |
| PYKK048 | Respiratory/1994 | Carrier | SC | + | +/+ | ++ |
| PYKK063 | Respiratory/1994 | Carrier | D | − | −/− | NA |
| PYKK065 | Respiratory/1994 | Carrier | D | − | −/− | NA |
| PYKK068 | Respiratory/1994 | Carrier | NS/NC | + | +/+ | − |
| PYKK069 | Respiratory/1994 | Carrier | NS/NC | + | +/+ | − |
| PYKK104 | Respiratory/1996 | Carrier | SC | + | +/+ | + |
| PYKK113 | Respiratory/1996 | Carrier | SC | + | +/+ | − |
| PYKK114 | Respiratory/1996 | Carrier | NS/NC | + | +/− | − |
| PYKK119 | Respiratory/1996 | Carrier | SC | + | +/+ | − |
| PYKK120 | Respiratory/1996 | Carrier | SC | + | +/+ | − |
| PYKK125 | Respiratory1997 | Carrier | SC | + | +/+ | − |
| PYKK200 | Respiratory/2004 | Carrier | SC | + | +/+ | + |
| PYO4a | Respiratory/2005 | Carrier | NS/NC | + | +/+ | − |
| PYP8 | Respiratory/2005 | Carrier | D | − | −/− | NA |
| PYKK070 | Skeletal/1993 | Osteomyelitis | D | − | −/− | NA |
| PYKK101 | Skeletal/1992 | Osteomyelitis | D | − | −/− | NA |
| PYKK141 | Skeletal/1996 | Osteomyelitis | NS/NC | + | +/+ | − |
| PYKK088 | Skeletal/1996 | Osteomyelitis | D | − | −/− | − |
| PYKK057 | Synovial/1995 | Septic arthritis | NS/NC | + | +/+ | − |
| PYKK058 | Synovial/1993 | Septic arthritis | D | − | −/− | NA |
| PYKK064 | Synovial/1994 | Septic arthritis | D | − | −/− | NA |
| PYKK077 | Synovial/1991 | Septic arthritis | D | − | −/− | NA |
| PYKK080 | Synovial/1996 | Septic arthritis | D | − | −/− | NA |
| PYKK081 | Synovial/1991 | Septic arthritis | NS/NC | + | +/+ | − |
| PYKK082 | Synovial/1990 | Septic arthritis | SC | + | +/+ | ++++ |
| PYKK083 | Synovial/1991 | Septic arthritis | D | − | −/− | NA |
| PYKK094 | Synovial/1995 | Septic arthritis | D | − | −/− | NA |
| PYKK100 | Synovial/1996 | Septic arthritis | SC | + | +/+ | + |
| PYKK102 | Synovial/1992 | Septic arthritis | D | − | −/− | NA |
| PYKK103 | Synovial/1992 | Septic arthritis | D | − | −/− | NA |
| PYKK123 | Synovial/1997 | Septic arthritis | NS/NC | + | +/+ | − |
| PYKK56 | Synovial/1994 | Septic arthritis | NS/NC | + | +/+ | − |
Colony types were designated spreading/corroding (SC), nonspreading/noncorroding (NS/NC), and domed (D).
Piliation was assessed by negative-staining transmission electron microscopy.
Adherence to Chang and Hig-82 cells was assessed by qualitative adherence assay.
Expression of PilA1 was assessed by Western blot analysis using antiserum GP65. The level of expression was compared to expression by strain 269-492, which was defined as ++++.
Strains PYHER, PYKK197, PYKK199, PY04a, PYP8, PYKK070, and PYKK057 were recovered from patients in central Israel, strains PYKK092, PYKK128, PYKK141, PYKK088, and PYKK064 were recovered from patients in northern Israel, and all other strains were recovered from patients in southern Israel. Strains PYKK060 and PYKK197 were recovered from adults, and all other strains were recovered from children between the ages of 6 months and 6 years.
Atypical short fibers were observed.
NA, not applicable.