TABLE 2.
Detection of protein-protein interactions in a yeast two-hybrid assay
| Fusion protein expressed from pGBKT7 | Interactiona with fusion protein expressed from pGADT7 |
|||||
|---|---|---|---|---|---|---|
| RsiA1 | RsiA2 | RpoE2 | RsiB1 | RsiB2 | None | |
| RsiA1 | − | − | ++ | ++ | + | − |
| RsiA2 | − | − | ++ | ++ | + | − |
| RpoE2 | ++ | + | − | − | − | − |
| RsiB1 | ++ | + | − | − | − | − |
| RsiB2 | ++ | ++ | − | − | − | − |
| None | − | − | − | − | − | − |
a
Shown is the ability (+) or not (−) of the AH109 strain containing the indicated pGADT7 and pGBKT7 derivatives to grow on plates of minimal medium lacking tryptophan, leucine, and histidine. ++ indicates that the strain was also able to grow on minimal medium lacking tryptophan, leucine, histidine, and adenine (higher stringency of the interaction test).