FIG. 2.

One-dimensional SW blot demonstrating DNA binding by rBCP. Blots were prepared from 1D SDS-PAGE gels (12.5% [wt/vol] acrylamide) with 5 μg of protein per well. (A) SW blot probed with biotin-labeled, C. burnetii genomic DNA. (B) SW blot duplicating that shown in panel A but done without a biotin-labeled DNA probe. Lanes: 1, biotinylated SDS-PAGE standards (Bio-Rad); 2, rBCP; 3, rCom1; 4, rHbpA; 5, Kaleidoscope prestained standards (Bio-Rad). The carbonic anyhdrase standard in lanes 5 (∼40.3 kDa) demonstrates intrinsic peroxidase activity and generates a signal on the SW blot regardless of whether or not a DNA probe is employed. Blot signals were detected using enhanced chemiluminescence (ECL) reagents and a 3-min exposure.