FIG. 3.

EMSA demonstrating DNA binding by rBCP. Components of a LightShift chemiluminescence EMSA kit were used as instructed by the manufacturer (Pierce). A comparison of mobilities for the biotin-EBNA DNA probe following acrylamide gel electrophoresis, blotting to nylon, and visualization of the signal is shown (see text for details). Lanes show the results of reactions performed with a biotin-EBNA DNA probe. Lanes: 1, no DNA-binding protein (negative control); 2, EBNA extract (positive control containing known DNA-binding protein); 3, heat-denatured (hd) EBNA extract; 4, 2 μM rBCP; 5, 10 μM rBCP; 6, 2 μM hd-rBCP; 7, 10 μM hd-rBCP. Sizes (indicated to the right in base pairs) were determined by the use of a GeneRuler biotinylated DNA ladder (KPL, Gaithersburg, MD).