FIG. 6.
Growth assays demonstrating functional expression of cloned Coxiella bcp and its ability to trans-complement E. coli bcp. Growth curves for an E. coli bcp mutant (LH200; •) and a trans-complemented daughter strain (LH100; ▪) are shown; the curve for the wild-type strain (LH300) was omitted for clarity. The upper lines (blue and green) depict growth in LBamp, while the lower lines (black and orange) show growth in an oxidative-stress growth medium (LBamp containing 0.2 mM t-BOOH). OD600 values represent the means of the results of 3 independent experiments ± standard deviations. Asterisks indicate a statistically significant (P < 0.05) difference between OD600 values at the indicated time points.