TABLE 1.
Bacterial strains, cell line, plasmids, and PCR primers used in the study
| Designation | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| C. burnetii | ||
| RSA 439 | Nine Mile Phase II, clone 4 | R. A. Heinzen |
| E. coli | ||
| DH5α | Host strain for plasmid production | Gibco-BRL |
| JM109 | Host strain for cloning | Promega |
| M15 (pREP4) | Host strain for His6 fusion proteins | Qiagen |
| JS2465-1 | bcp-728Δ::kan | CGSC (3) |
| BW25113 | Parent strain to JS2465-1 | CGSC (3) |
| LH001 | In-frame bcp mutant derived from JS2465-1 | This study |
| LH100 | LH001 containing pQBCP | This study |
| LH200 | LH001 containing pQE30 | This study |
| LH300 | BW25113 containing pQE30 | This study |
| LH400 | M15 (pREP4) containing pQE30 | This study |
| LH500 | M15 (pREP4) containing pQBCP | This study |
| LH600 | M15 (pREP4) containing pQCOM1 | This study |
| LH700 | M15 (pREP4) containing pQHBPA | This study |
| Cell line | ||
| Vero | Green monkey epithelial line | ATCC CCL-81 |
| Plasmids | ||
| pUC19 | High-copy-number cloning vector | 30 |
| pCP20 | flp+, Repts, Apr, Cmra | 5 |
| pQE30, 31 | Expression vectors for His6 fusion proteins | Qiagen |
| pREP4 | lacI+ in trans repressor plasmid | Qiagen |
| pQBCP | pQE30 with cloned Coxiella bcp and sdr | This study |
| pQCOM1 | pQE30 with cloned Coxiella com1 | This study |
| pQHBPA | pQE31 with cloned Bartonella quintana hbpA | This study |
| PCR primers | ||
| BCP_For | GCGCATGCTCCATTGAAGTCGGCCAGAAGG | This study |
| BCP_Rev | TCGCTGCAGGCTTGGTTAGCTGAACATAC | This study |
| BCP2_For | TGAATGATTGATGTGCTTTAACG | This study |
| BCP2_Rev | CGGATGAAGGCGAAATGC | This study |
a
Ap, ampicillin; Cm, chloramphenicol; ts, temperature sensitive; s, susceptible.