TABLE 2.
Isolated plasmids containing linker insertions
| No. isolated | Plasmid | Linker position in cI | Position of stop codon or linker insertion in CI | Mean promoter activity (Miller units) ± SDa |
|
|---|---|---|---|---|---|
| PL | PR | ||||
| 1 | pML37 | 30 | TAA at 12 | ND | ND |
| 1 | pML2 | 36 | TAA at 14 | ND | ND |
| 1 | pML10 | 48 | TAA at 18 | ND | ND |
| 1 | pML11 | 49 | TAA at 18 | ND | ND |
| 1 | pML15 | 162 | TAA at 56 | ND | ND |
| 1 | pML5 | 192 | TAA at 66 | ND | ND |
| 1 | pML26 | 227 | TAA at 78 | ND | ND |
| 1 | pML3 | 285 | TAA at 96 | ND | ND |
| 1 | pML25 | 317 | TAA at 108 | ND | ND |
| 1 | pML39 | 351 | TAA at 119 | ND | ND |
| 1 | pML30 | 8 | 3 | 141 ± 15 | ND |
| 1 | pML21 | 19 | 6 | 0.7 ± 0.2 | ND |
| 1 | pML19 | 31 | 11 | 778 ± 25 | ND |
| 1 | pML7 | 41 | 14 | 892 ± 40 | ND |
| 2 | pML17/pML18 | 44 | 15 | 1,095 ± 28 | ND |
| 1 | pML28 | 62 | 20 | 1,081 ± 154 | ND |
| 1 | pML34 | 124 | 41 | 861 ± 163 | ND |
| 2 | pML13/pML14 | 227 | 75 | 243 ± 19 | ND |
| —b | pMAP150 | 233 | 78 | 1.3 ± 0.4 | 2.0 ± 0.1 |
| —b | pMAP151 | 257 | 89 | 0.8 ± 0.3 | 1.5 ± 0.2 |
| 1 | pML8 | 295 | 98 | 0.6 ± 0.2 | 1.4 ± 0.1 |
| 2 | pML9/pML38 | 307 | 101 | 1.4 ± 0.1 | 10.7 ± 1 |
| 1 | pML35 | 340 | 113 | 1.1 ± 0.1 | 6.6 ± 2 |
| 1 | pML6 | 358 | 120 | 1.1 ± 0.3 | 7.7 ± 0.4 |
| 1 | pML32 | 362 | 121 | 1.3 ± 0.1 | 5.2 ± 0.3 |
| 1 | pML33 | 368 | 122 | 0.6 ± 0.1 | 2.1 ± 0.2 |
| 2 | pML27/pML36 | 406/407 | 135 | 0.3 ± 0.1 | 1.2 ± 0.1 |
| None | pAJ80 | 1.0 ± 0.3 | 1.3 ± 0.1 | ||
| None | pCI372 | No cI | 1,023 ± 38 | 34 ± 5 | |
a
A420/(ml·min·OD600). ND, not determined.
b
—, the 15-base-pair linker was obtained not by using the GPS-LS linker scanning system but by site-directed mutagenesis.