FIG. 1.

Expression and steady-state levels of SodCI and SodCII in vivo. Strains contain a chromosomal lacZ fusion in which the reporter gene is inserted just downstream of the stop codon for either sodCI-6xhis or sodCII. (A) Bacteria were recovered from LB after 16 h or from macrophages (MΦ) after 16 h of infection. (B) Bacteria were recovered from LB after 16 h or from mouse spleens (M) after 4 days of infection. (C) Same as in panel A, except that the background strain was a ΔhtrA mutant. Whole-cell extracts were prepared from equal numbers of bacteria, separated by SDS-PAGE, and subjected to immunoblot analysis. Each set of panels represents a single gel for which the resulting nitrocellulose membrane was cut into sections and processed with primary antibody directed against the indicated protein. These results are representative of experiments performed 2 to 6 times. The strains used were JS905, JS906, and JS907.