Transcription of sodCII is regulated by RpoS but is not directly affected by zinc level. (A) β-Galactosidase activity in strains containing sodCII-lacZ or katE-lacZ fusion in wild-type (WT) or rpoS null background, grown overnight in LB. The strains used were JS531, JS908, JS909, and JS910. (B) β-Galactosidase activity in strains containing lacZ fusions to the indicated genes in znuA+ or znuA null background, grown overnight in LB containing either EDTA or the zinc chelator TPEN, as indicated. The strains used were JS911, JS531, JS912, JS909, and JS913. (C) β-Galactosidase activity in strains containing znuA-lacZ or sodCII-lacZ fusion in wild-type or zur null background, grown overnight in LB. The strains used were JS911, JS914, JS531, and JS915.