TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotypea | Deletion or cloned endpointsb | Source or referencec |
|---|---|---|---|
| Strains | |||
| 14028 | Wild type | ATCC | |
| JSG435 | pmrA505 zjd::Tn10d-Cam | 16 | |
| JSG1050 | pmrI::MudJ | 43 | |
| TN3740 | LT2 leu-485 trp-190::(lacI lacp T7 pol spc) | C. G. Miller | |
| JS531 | Φ(sodCII+-lac+)110 | 1516586-1516598 | 13 |
| JS900 | Φ(sodCI+-6xhis-lac+)2920 | 1098178-1130040 | |
| JS901 | ΔhtrA::Kan | 244465-245933 | |
| JS902 | ΔkatE11::Cam | 1397114-1399406 | |
| JS903 | Δzur::Cam | 4461801-4462315 | |
| JS904 | ΔoppA-F::Cam | 1858868-1841314 | |
| JS905 | Φ(sodCI+-6xhis-lac+)2920 zyd::Tn10d-Cam | ||
| JS906 | Φ(sodCII+-lac+)110 zyd::Tn10d-Cam | ||
| JS907 | Φ(sodCII+-lac+)110 ΔhtrA::Kan zyd::Tn10d-Cam | ||
| JS908 | Φ(sodCII+-lac+)110 ΔrpoS1191::Tet | ||
| JS909 | Φ(katE-lac+)11 | ||
| JS910 | Φ(katE-lac+)11 ΔrpoS1191::Tet | ||
| JS911 | Φ(znuA-lac+) | ||
| JS912 | Φ(sodCII+-lac+)110 ΔznuA::Cam | ||
| JS913 | Φ(katE-lac+)11 ΔznuA::Cam | ||
| JS914 | Φ(znuA-lac+) Δzur::Cam | ||
| JS915 | Φ(sodCII+-lac+)110 Δzur::Cam | ||
| JS916 | ΔsodCI-104::Cam ΔsodCII-103::Kan/pMC101 | ||
| JS917 | ΔsodCI-104::Cam ΔsodCII-103::Kan/pMC102 | ||
| JS918 | ΔznuA::Cam | 1986415-1987359 | |
| JS921 | ΔoppA-F::Cam trp190::(lacI lacp T7 pol spc) ΔsodA101 ΔsodB102 ΔsodCI-104 ΔsodCII-103/pBK103 | ||
| JS922 | ΔsodA101 ΔsodB 102 ΔsodCI::Tet ΔsodCII-105::Cam/pMC102 | ||
| JS923 | ΔphoN::Kan/pBK104 | ||
| JS924 | ΔsodA101 ΔsodB102 ΔsodCI::Tet ΔsodCII-105::Cam/pMC101 | ||
| JS925 | ΔsodA101 ΔsodB102 ΔsodCI::Tet ΔsodCII-105::Cam/pRK106 | ||
| JS926 | zjd::Tn10d-Cam/pMC101 | ||
| JS927 | pmrA505 zjd::Tn10d-Cam/pMC101 | ||
| JS928 | pmrI::MudJ | ||
| JS929 | pmrI::MudJ pmrA505 zjd::Tn10d-Cam | ||
| JS930 | Φ(sodCII+-lac+)110 pmrA505 zjd::Tn10d-Cam | ||
| JS931 | ΔsodCI-104::Kan zjd::Tn10d-Cam | ||
| JS932 | ΔsodCI-104::Kan sodCII-106::Tet zjd::Tn10d-Cam | ||
| JS933 | ΔsodCI-104::Kan pmrA505 zjd::Tn10d-Cam | ||
| JS934 | ΔsodCI-104::Kan ΔsodCII-106::Tet pmrA505 zjd::Tn10d-Cam | ||
| JS935 | ΔsodCII-103::Kan | ||
| JS936 | ΔsodCI-104::Cam ΔsodCII-103::Kan | ||
| Plasmids | |||
| pBK103 | pET21b sodCI-6xhis | 1130056-1130586 | |
| pMC101 | pWKS30 sodCI | 21 | |
| pMC102 | pWSK29 sodCII | 21 | |
| pRK106 | pWKS30 sodCI Y87E Y109E | 22 | |
| pBK104 | pWSK30 phoA-6xhis | ||
| pKD46 | 6 | ||
| pCP20 | 6 | ||
| pKG136 | 8 |
a
All Salmonella strains are isogenic derivatives of Salmonella serovar Typhimurium strain 14028.
b
Numbers indicate the base pairs that are deleted or cloned (inclusive), as defined in the S. enterica serovar Typhimurium LT2 genome sequence (National Center for Biotechnology Information).
c
This study unless specified otherwise. ATCC, American Type Culture Collection.