FIG. 1.

The ΔflgT mutant is defective in attachment and colonization. (A) V. cholerae strain O395 (WT) and ΔflaA and ΔflgT mutants were competed against an O395 ΔlacZ strain (KSK258) for in vitro attachment to HT-29 epithelial cells. Statistical analyses (unpaired Student's t test) were performed between each mutant and the WT, as indicated by asterisks. ***, P < 0.0001. (B) V. cholerae strain O395 (WT) and ΔflaA and ΔflgT mutants were competed against an O395 ΔlacZ strain (KSK258) for in vivo colonization of infant mice, using four to seven mice per group. Statistical analyses (unpaired Student's t test) were performed between each mutant and the WT, as indicated by asterisks. ***, P < 0.0001.