FIG. 2.

FlgT is essential for motility and flagellar stability. (A) Zones of motility of V. cholerae WT, ΔflaA, ΔflgT, and ΔflgT/pFlgT-His₆ strains in motility agar. (B) Transmission electron micrographs showing the polar flagella of the V. cholerae WT (i) and the ΔflgT strains. The two ΔflgT mutant cell types are shown in panels ii (flagellate) and iii (aflagellate). Grids were prepared at an OD₆₀₀ of ∼2.0. Bar, 500 nm. (C) Graphical representation of percent flagellated cells of the V. cholerae WT, ΔflaA, and ΔflgT strains during mid-log (OD₆₀₀ = 0.5), late-log (OD₆₀₀ = 2.0), and stationary (OD₆₀₀ = 4.0) phases. A total of 100 cells per strain were examined via transmission electron microscopy. Brackets indicate statistical comparisons (unpaired Student's t test) between the indicated data sets. ***, P < 0.0001; *, P < 0.05.