TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli strains | ||
| S17-1λpir | Tpr SmrrecA thi pro hsdR M+ [RP4-2Tc::Mu::Kmr Tn7] (λpir) | 34; laboratory collection |
| Top-10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| V. cholerae strains | ||
| O395 | Classical Ogawa strain; Smr | Laboratory collection |
| KSK258 | O395 ΔlacZ3 | Karen Skorupski |
| BAJ43 | O395 ΔflgT | This study |
| BAJ61 | BAJ43(pBro26) | This study |
| RM414 | BAJ43(pBro37) | This study |
| RM421 | BAJ43(pBAD202) | This study |
| TJK189 | O395 ΔflaA | This study |
| Plasmids | ||
| pBAD202 | Kmr | Invitrogen |
| pBAD-TOPO | Ampr | Invitrogen |
| pBro26 | pBAD-TOPO FlgT-His6 | This study |
| pBro32 | pKAS32 ΔflgT | This study |
| pBro37 | pBAD202 FlgT-His6 | This study |
| pKAS32 | Allelic exchange vector; Ampr | 37 |
| pTK20 | pKAS32 ΔflaA | This study |