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Structure of lipid A from O1 V. cholerae (A) and CAD tandem mass spectra of LApenta-PO4H3 (B), LAtetra (C), and LAtri (D) from C6706 ΔmsbB. A collision cell voltage of 30 to 40 V was used to induce fragmentation of mass-selectively accumulated precursor ions. Observed bond cleavages are summarized in panel A. Similar results were obtained from tandem mass spectrometry of C6706, O395, and O395 ΔmsbB LPS anions (data not shown). Precursor ions are marked with asterisks.
