FIG. 5.

The W/Beijing frameshift mutation renders DosT nonfunctional. qRT-PCR analysis of dosR (A), Rv2626c (B), and dosT (C) expression levels in group 1 W/Beijing (1-3; white bars), group 4 W/Beijing (G4B1.2; gray bars), and H37Rv (black bars) parental and recombinant strains expressing either full-length wild-type (pPDM11) or full-length mutant (pdosTmut) dosT under the control of the hsp60 promoter in the pMV361 vector. Expression levels for both DETA-NO-treated (hatched bars) and untreated (solid bars) samples are presented and are normalized to levels of the sigA housekeeping gene. Representative data for at least two independent biological replicates are shown, with each sample assayed in triplicate. Error bars represent standard deviations. Note that different scales were used for panels A, B, and C.