FIG. 10.

Seemingly independent localization of septins and LEPs. (A) Localization of Spn2 and Psy1 in meu14Δ cells. Strain SY211 (spn2⁺-mRFP GFP-psy1⁺ meu14Δ) was sporulated and observed without fixation. (B) Localization of Meu14 in spnΔ cells. Strains SY13 (meu14⁺-GFP), SY86 (spn2Δ meu14⁺-GFP), SY288 (spn5Δ meu14⁺-GFP), SY284 (spn6Δ meu14⁺-GFP), and SY292 (spn7Δ meu14⁺-GFP) were sporulated, fixed, and stained with DAPI. Representative cells in anaphase II (wild type and spn2Δ mutant) or metaphase (spn5Δ, spn6Δ, and spn7Δ mutants) are shown.