TABLE 3.
Expt | Strain | Genotype | % Asci with four spores | % Asci with no spores |
---|---|---|---|---|
1 | THP18 | Wild type | 85 | 2 |
SY154 | meu14Δ | 65 | 14 | |
MO684 | spn2Δ | 57 | 5 | |
SY202 | spn2Δ meu14Δ | 32 | 21 | |
SY233 | spn5Δ | 17 | 11 | |
SY430 | spn5Δ meu14Δ | 5 | 62 | |
SY175 | spn7Δ | 21 | 20 | |
SY386 | spn7Δ meu14Δ | 3 | 71 | |
2 | MO670 | Wild type | 89 | 9 |
MO905 | meu14Δ | 25 | 20 | |
MO667 | spn6Δ | 4 | 34 | |
MO911 | spn6Δ meu14Δ | 1 | 69 |
Data are from two separate experiments. In experiment 1, cells of the indicated strains were incubated on an SSA plate at 30°C for 2 days and examined by phase-contrast microscopy using an Olympus microscope. In experiment 2, cells of the indicated strains were incubated on an MM-N plate at 30°C for 1 day and examined by DIC microscopy using a Nikon Eclipse microscope.