FIG. 5.

Additional level of redundancy in the C-terminal tail: functional relevance of Grb2 binding and tyrosines of the novel motifs in Dof. Mutant full-length (FL) Dof constructs were tested for their ability to rescue defects in the development of the mesoderm and the tracheae in dof mutant embryos. Grb2F, amino acid exchange of the tyrosines of all four consensus Grb2 recognition sites at positions Y914, Y930, Y988, and Y1009 to phenylalanines. 844AA and 891AA, an amino acid exchange of the two conserved prolines to alanines in the two novel motifs with tyrosine positions 844 and 891. #, reasonable rescue of dorsal and lateral tracheal branches despite a weak rescue of dorsal trunk fusions. For further details, see Fig. 3.