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. 2010 Feb 1;30(8):1997–2005. doi: 10.1128/MCB.01116-09

FIG. 5.

FIG. 5.

LSD1 siRNA treatment leads to reduced cell proliferation in the dentate gyri of adult mouse brains. (a) Expression of LSD1 in the subgranular zone (SGZ) of the dentate gyri of wild-type adult mouse brains as revealed by immunostaining. The LSD1 staining is shown in red. Cells indicated by arrows are examples of the LSD1-positive cells in the SGZ. An enlarged image of the LSD1-positive cells in the boxed region is shown on the right. (b) Knockdown of LSD1 expression using the LSD1 siRNA-expressing lentivirus in cultured neural stem cells. SC, scrambled siRNA control. Actin was included as a loading control. (c) Reduced BrdU staining of LSD1 siRNA-transduced cells in the dentate gyri (DG) of adult brains. DG sections from scrambled siRNA control- or LSD1 siRNA-transduced and BrdU-treated mice were immunostained for BrdU (blue). NeuN staining (red) was included to show the structure of the DG. The virus-transduced cells are shown in green due to the expression of a GFP marker. (d) Percentages of doubly GFP-positive and BrdU-positive cells out of GFP+ cells in the dentate gyrus SGZ of the LSD1 siRNA lentivirus-infected mice. SC, scrambled control siRNA; siLSD1, LSD1 siRNA. Data are represented as means ± standard deviations. *, P < 0.01 by Student's t test. (e) Examples of scrambled siRNA control (sc siRNA-GFP)- or LSD1 siRNA (siLSD1-GFP)-transduced cells in the dentate gyrus (DG) SGZ. LSD1 staining is shown in red. The virus-transduced cells are shown in green due to the expression of a GFP marker. The merged images are shown on the right.